Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time
Odontologia

Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time


Abstract
Introduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. Article published in: RSBO. 2012 Jan-Mar;9(1):67-73.

Download full text.




loading...

- Anatomical Variations In The Permanent Mandibular Canine: Forensic Importance
Abstract.Introduction: The uniqueness of dental morphology plays an important role in Forensic Odontology, especially for human identifications, in which a single tooth can provide information  for dental identification. Objective: To...

- Comparative Study Among Dentistry Undergraduates And Forensic Odontology Postgraduate Students Through Smile Photographs For Human Identification
Abstract. Introduction: The execution of forensic odontology technique for human identification depends on the existence of dental files produced ante-mortem (dental records, clinical notes, radiographs or dental casts). However, when...

- Identification Of A Charred Corpse Through Dental Records
AbstractIntroduction: Forensic Dentistry is an area of Dentistry related to Law. Among its goals is the execution of human identification, defined as the process of determining the person’s identity. The forensic dentist uses, in this process, ante-mortem...

- Survival Of Batch Numbers Within Dental Implants Following Incineration As An Aid To Identification
Abstract Dental implants have become a popular choice of treatment in replacing individual lost teeth or entire dentitions. The physical properties of high corrosion resistance, high structural strength and high melting point, suggest the retention of...

- Genetics And Molecular Biology: A Literature Review Of Forensic Dentistry Application
AbstractForensic expertise methodology normally used in different criminal investigation and forensic medicine field such as blood type, anthropologic analysis and forensic dentistry (dental records, X-rays, bite marks, among others) solved and will...



Odontologia








.